MACS SmartStrainer, 100 µM from Miltenyi Biotec

(1) Single-cell isolation from full-thickness human intestinal tissue resections for single-cell RNA sequencingSTAR ProtocolsDecember 1, 2023Gail A. West, Shuai Zhao, Quang Tam Nguyen, Stephen M. Christensen, Ilyssa O. Gordon, Stefan D. Holubar, Kellie M. Kravarik, Claudio Fiocchi, Pranab K. Mukherjee, Florian Riedersolution])",BD Biosciences,556547 ,, Other,Other,Other ,, "Cell strainer, 100 μm",Miltenyi Biotec,130-098-463 Corning 60 mm cell culture plate,Corning,430166 100 mm dishes,Falcon,351029 "Curved, round-ended

(2) Expansion of CD4+ cytotoxic T lymphocytes with specific gene expression patterns may contribute to suppression of tumor immunity in oral squamous cell carcinoma: single-cell analysis and in vitro experimentsFrontiers in ImmunologyNovember 23, 2023Hu Chen, Junsei Sameshima, Shiho Yokomizo, Tomoki Sueyoshi, Haruki Nagano, Yuka Miyahara, Taiki Sakamoto, Shinsuke Fujii, Tamotsu Kiyoshima, Thomas Guy, et al.Following homogenization for 1 hour, the tissue sample was passed through a 100 µm MACS SmartStrainer (130-098-463, Miltenyi Biotec) and transferred into a 5 mL Round Bottom Polystyrene Test Tube with Cell Strainer

(3) Toll-like receptor 9-positive plasmacytoid dendritic cells promote Th17 immune responses in oral lichen planus stimulated by epithelium-derived cathepsin KScientific ReportsNovember 7, 2023Yuka Miyahara, Hu Chen, Masafumi Moriyama, Keita Mochizuki, Naoki Kaneko, A. S. M. Rafiul Haque, Akira Chinju, Kazuki Kai, Mizuki Sakamoto, Noriko Kakizoe-Ishiguro, et al.aggregates or large particles in cell suspension were removed by MACS SmartStrainers (100 μm) (Catalog No. 130-098-463; Miltenyi Biotec) then the cell suspension was filtered into the 5 ml Polystyrene Round-Bottom Tube

(4) Protocol for generating airway organoids from 2D air liquid interface-differentiated nasal epithelia for use in a functional CFTR assaySTAR ProtocolsSeptember 1, 2023Lisa W. Rodenburg, Isabelle S. van der Windt, Henriette H.M. Dreyer, Shannon M.A. Smits, Loes A. den Hertog - Oosterhoff, Ellen M. Aarts, Jeffrey M. Beekman, Gimano D. AmatngalimDiagnostics,Cat#43-50030 pluriStrainer® 100 μm,ITK Diagnostics,Cat#43-57100-51 MACS SmartStrainers (100 μm),Miltenyi Biotec bv,Cat#130-098-463 12-mm Transwell with 0.4-μm Pore Polyester Membrane Insert,Corning,Cat#3460 6.5-mm Transwell with

(5) Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantationNature communicationsJune 13, 2023Firl DJ, Lassiter G, Hirose T, Policastro R, D'Attilio A, Markmann JF, Kawai T, Hall KC.The resulting single-cell suspensions were passed through a 100 µm cell strainer (Miltenyi Biotec, 130-098-463), centrifuged and then passed through a 70 µm cell strainer (Miltenyi Biotec, 130-098-462) and centrifuged

(6) Zebrafish pigment cells develop directly from persistent highly multipotent progenitorsNature CommunicationsMarch 6, 2023Tatiana Subkhankulova, Karen Camargo Sosa, Leonid A. Uroshlev, Masataka Nikaido, Noah Shriever, Artem S. Kasianov, Xueyan Yang, Frederico S. L. M. Rodrigues, Thomas J. Carney, Gemma Bavister, et al.Pasteur pipette; passed through 100-micron strainer (MACS SmartStrainers, Miltenyi Biotech., Cat. No. 130-098-463,) into 50 ml Falcon tube and centrifuged for 5 min, 500 x g, 4 °C. The cell pellet was re-suspended

(7) The lysophosphatidic acid-regulated signal transduction network in ovarian cancer cells and its role in actomyosin dynamics, cell migration and entosisTheranosticsJanuary 1, 2023Ojasalu K, Lieber S, Sokol AM, Nist A, Stiewe T, Bullwinkel I, Finkernagel F, Reinartz S, Müller-Brüsselbach S, Grosse R, et al.obtained from ascites by consecutive filtration using 30 and 100 µm cell strainers (130-098-458 and 130-098-463, Miltenyi Biotech, Bergisch Gladbach, Germany). The cells were fixed with 4% formaldehyde, embedded

(8) Culture media composition influences patient-derived organoid ability to predict therapeutic responses in gastrointestinal cancersJCI InsightNovember 22, 2022Tara L. Hogenson, Hao Xie, William J. Phillips, Merih D. Toruner, Jenny J. Li, Isaac P. Horn, Devin J. Kennedy, Luciana L. Almada, David L. Marks, Ryan M. Carr, et al.gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec), filtered through a MACS SmartStrainer (100 μm) filter (Miltenyi Biotec, 130-098-463), and cells were plated in 1 well of 12-well flat-bottom cell culture plate (Corning, 3513) coated with

(9) Multiparametric Flow Cytometry-Based Immunophenotyping of Mouse Liver Immune CellsMethods and ProtocolsSeptember 3, 2022Lenka Vanekova, Marketa Pimkova Pimkova Polidarova, Vaclav Veverka, Gabriel Birkus, Andrea BrazdovaCat. no.: L12003)MACS SmartStrainer, 100 µm (Miltenyi Biotec, Bergisch Gladbach, Germany; Cat. no.: 130-098-463)Neo Delta Ven T cannula 24G (Delta Med, Viadana, Lombardia, Italy; Cat. no.: 3113122)R540 Enhanced Anesthesia

(10) Mass cytometry staining for human bone marrow clinical samplesSTAR protocolsMarch 18, 2022Hallisey M, Dennis J, Abrecht C, Pistofidis RS, Schork AN, Lightbody ED, Heilpern-Mallory D, Severgnini M, Ghobrial IM, Hodi FS, et al.#1367530 25 mL Serological Pipet,Corning Inc.,Cat #1367530 MACS SmartStrainer 100 μm,Miltenyi Biotec,Cat #130098463 autoMACS Pro,Miltenyi Biotec,Cat #130092545 CoolCell FTS30,Thermo Fisher ScientificTM,Cat #07210008 Countess II Automated Cell Counter

(11) Single-cell RNA sequencing uncovers the individual alteration of intestinal mucosal immunocytes in Dusp6 knockout miceiScienceFebruary 18, 2022Chang CS, Yu WH, Su CC, Ruan JW, Lin CM, Huang CT, Tsai YT, Lin IJ, Lai CY, Chuang TH, et al.2017)",https://evol.nhri.org.tw/phenome2/ ,, Other,Other,Other ,, gentleMACS Dissociator,Miltenyi Biotec,Cat#130-093-235 100 μm strainer,Miltenyi Biotec,Cat#130-098-463 70 μm strainer,FALCON,Cat#352350 cell sorter,BD Biosciences,BD Influx cell counter,Nexcelom Bioscience,Cellometer

(12) Cell-type-specific signaling networks in heterocellular organoidsNature methodsMarch 1, 2020Qin X, Sufi J, Vlckova P, Kyriakidou P, Acton SE, Li VSW, Nitz M, Tape CJ.the pellet was resuspended in DMEM + 10% FBS (Thermo 10082147). Cells were 100 μm filtered (Miltenyi 130-098-463) into a T75 flask and incubated at 5% CO2, 37 °C. After 3 hrs, cells were washed ×2 with PBS to remove

(13) A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic SyndromeCell reportsDecember 11, 2018Su S, Guntur AR, Nguyen DC, Fakory SS, Doucette CC, Leech C, Lotana H, Kelley M, Kohli J, Martino J, et al.dish,CytoOne,Cat#CC7682-3394 5 ml Polystyrene round-bottom tube,Corning,Cat#352054 MACS SmartStrainers (100 μm),Miltenyi,Cat#130-098-463 Microcentrifuge Tubes (1.7 ml),National Scientific Supply Co,Cat#CA1700-BP Seahorse XF24 V7 PS Cell Culture

(14) Tumor-immune profiling of murine syngeneic tumor models as a framework to guide mechanistic studies and predict therapy response in distinct tumor microenvironmentsPloS oneJanuary 1, 2018Yu JW, Bhattacharya S, Yanamandra N, Kilian D, Shi H, Yadavilli S, Katlinskaya Y, Kaczynski H, Conner M, Benson W, et al.syngeneic tumor models tumors were filtered through 100μM & 30μM pre-separation filters (Miltenyi #130-098463,130-098-458), washed with PBS, and then used for flow cytometry or RNA based analysis. For each sample,

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MACS SmartStrainers (100 µm), 50 filters (sterile, single-packed) from Miltenyi Biotec

MACS SmartStrainer, 100 µM from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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MACS SmartStrainer, 100 µM from Miltenyi Biotec

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